In vitro reconstitution of complexes of stress HliA protein with pigments

Proteins similar to Hli (high light inducible) proteins of cyanobacteria are present in all photosynthetic eukaryotes and necessary for survival various stressful conditions, although their exact function is not fully understood. In current study, the recombinant stress-induced protein HliA cyanobacterium Synechocystis was isolated characterised first time. The synthetic gene created cloned into plasmid expression with Hisx6-tag at C-terminus bacteria. Recombinant by metal-affinity chromatography. reconstituted chlorophyll a carotenoids. Using circular dichroism spectroscopy, it shown that carotenoids interact vitro protein. binding pigments favours protective this Apparently, involved coordinated delivery biogenesis complexes, thereby reducing risk accumulation phototoxic free molecules. Current results important understanding processes photoprotection either or algae higher plants.